A hotspot for transition mutations in the rIIB gene of bacteriophage T4
Identifieur interne : 00EC50 ( Main/Exploration ); précédent : 00EC49; suivant : 00EC51A hotspot for transition mutations in the rIIB gene of bacteriophage T4
Auteurs : Britta Swebilius Singer [États-Unis]Source :
- Molecular and General Genetics MGG [ 0026-8925 ] ; 1984-01-01.
English descriptors
- Teeft :
- Bacteriophage, Benzer, Biol, Champe, Coding region, Codon, Cytosine, Deletion, Detoxifying, Detoxifying mutations, Dispensable region, Frameshift, Frameshift mutation, Frameshift mutations, Frameshifts, Guanine, Hotspot, Hydroxylamine, Lysogen, Missense, Missense mutations, Mutability, Mutable, Mutant, Mutation, Napiv2, Nitrous, Nitrous acid, Nonsense mutations, Plaque, Point mutations, Polypeptide, Pribnow, Pseudowild, Recombinant, Recombine, Riia, Riib, Tabr3, Tggcaa, Wild type sequence.
Abstract
Summary: We have previously demonstrated that the sequence 5′TGGCAA 3′ located at codons 32–33 of the rIIB gene of bacteriophage T4 is a hotspot for transition mutations (Nelson et al. 1981). Here I report the properties of the same TGGCAA sequence introduced into the gene at codons 11–12. The sequence is highly mutable in both locations, suggesting that its high mutability is due to features of the TGGCAA itself and is not dependent on the immediate juxtaposition of additional external sequences. Within this sequence, at either location, only the transition at the central G:C pair frequently arises spontaneously or by 2-aminopurine or ethylmethane sulfonate mutagenesis. However, the 3′ G:C pair, in addition, is highly mutable after nitrous acid or hydroxylamine treatment. This suggests that, within the TGGCAA sequence, there are two hotspots which are targeted by different mutagens.
Url:
DOI: 10.1007/BF00327422
Affiliations:
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Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">A hotspot for transition mutations in the rIIB gene of bacteriophage T4</title><author><name sortKey="Singer, Britta Swebilius" sort="Singer, Britta Swebilius" uniqKey="Singer B" first="Britta Swebilius" last="Singer">Britta Swebilius Singer</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:1BCA4327C67BF00D059FBBF9F451FAD0AAB6AD4B</idno><date when="1984" year="1984">1984</date><idno type="doi">10.1007/BF00327422</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-R7XTH1WQ-M/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000626</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000626</idno><idno type="wicri:Area/Istex/Curation">000621</idno><idno type="wicri:Area/Istex/Checkpoint">003777</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">003777</idno><idno type="wicri:doubleKey">0026-8925:1984:Singer B:a:hotspot:for</idno><idno type="wicri:Area/Main/Merge">00F540</idno><idno type="wicri:Area/Main/Curation">00EC50</idno><idno type="wicri:Area/Main/Exploration">00EC50</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">A hotspot for transition mutations in the rIIB gene of bacteriophage T4</title><author><name sortKey="Singer, Britta Swebilius" sort="Singer, Britta Swebilius" uniqKey="Singer B" first="Britta Swebilius" last="Singer">Britta Swebilius Singer</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular, Cellular and Developmental Biology, University of Colorado, 80309, Boulder, Colorado</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular and General Genetics MGG</title><title level="j" type="abbrev">Molec. Gen. Genet.</title><idno type="ISSN">0026-8925</idno><idno type="eISSN">1432-1874</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1984-01-01">1984-01-01</date><biblScope unit="volume">193</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="104">104</biblScope><biblScope unit="page" to="109">109</biblScope></imprint><idno type="ISSN">0026-8925</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0026-8925</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Bacteriophage</term><term>Benzer</term><term>Biol</term><term>Champe</term><term>Coding region</term><term>Codon</term><term>Cytosine</term><term>Deletion</term><term>Detoxifying</term><term>Detoxifying mutations</term><term>Dispensable region</term><term>Frameshift</term><term>Frameshift mutation</term><term>Frameshift mutations</term><term>Frameshifts</term><term>Guanine</term><term>Hotspot</term><term>Hydroxylamine</term><term>Lysogen</term><term>Missense</term><term>Missense mutations</term><term>Mutability</term><term>Mutable</term><term>Mutant</term><term>Mutation</term><term>Napiv2</term><term>Nitrous</term><term>Nitrous acid</term><term>Nonsense mutations</term><term>Plaque</term><term>Point mutations</term><term>Polypeptide</term><term>Pribnow</term><term>Pseudowild</term><term>Recombinant</term><term>Recombine</term><term>Riia</term><term>Riib</term><term>Tabr3</term><term>Tggcaa</term><term>Wild type sequence</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: We have previously demonstrated that the sequence 5′TGGCAA 3′ located at codons 32–33 of the rIIB gene of bacteriophage T4 is a hotspot for transition mutations (Nelson et al. 1981). Here I report the properties of the same TGGCAA sequence introduced into the gene at codons 11–12. The sequence is highly mutable in both locations, suggesting that its high mutability is due to features of the TGGCAA itself and is not dependent on the immediate juxtaposition of additional external sequences. Within this sequence, at either location, only the transition at the central G:C pair frequently arises spontaneously or by 2-aminopurine or ethylmethane sulfonate mutagenesis. However, the 3′ G:C pair, in addition, is highly mutable after nitrous acid or hydroxylamine treatment. This suggests that, within the TGGCAA sequence, there are two hotspots which are targeted by different mutagens.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Colorado</li></region></list><tree><country name="États-Unis"><region name="Colorado"><name sortKey="Singer, Britta Swebilius" sort="Singer, Britta Swebilius" uniqKey="Singer B" first="Britta Swebilius" last="Singer">Britta Swebilius Singer</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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